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Protocols
& Methods
Herskowitz Lab Protocols
(UCSF) is a large
collection of methods for
S. cerevisiae genetics, cell biology andochemistry...-
DNA Protein Analysis Toolkit
Computing Services from The Rockefeller
University, a compilation of molecular biology Web links
in a format based on function....
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CMS-SDSC Molecular Biology Resource
Internet resources for molecular biology,
biotechnology, biomolecular modeling...
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LabConsumer
a section of The Scientist Journal that
presents updated information about biological methods
and discusses specific research tools used in the laboratory....
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David Bowtell's Biochemistry, Molecular Biology and
Cell Biology Protocols collection
of protocols in biochemistry, molecular biology and
cell biology, include In-Situ hybridisation, Extraction
and Purification of Eurkaryotic RNA, Analysis of Eukaryotic
RNA...
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Brendan Babb's Collection of Protocols collection
of molecular biology techniques, tips, recipes and protocols
arranged in alphabetical order...
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Helen Donis-Keller Laboratory Manual includes
Lambda Phage Propagation and DNA Preparation Methods,
Plasmid/Cosmid Propagation and DNA Preparation, Yeast
Artificial Chromosome (YAC)Methods, Human DNA Preparations,
Restriction Digestion Methods, Southern Transfer and
Hybridization, DNA Sequencing, PCR Methods, Denaturing
Gradient Gel Electrophoresis (DGGE), Human Cell Culture
Methods, Chromosome Analysis Methods...
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PCR and multiplex PCR: guide and troubleshooting
by Octavian Henegariu at Indiana University,
PCR techniques and troubleshooting...
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BioGuide - PCR
at the Weizmann Institute of Science
Genome and
Bioinformatics Unit, a resource for PCR techniques and
for programs to design PCR primers...
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The Polymerase Chain Reaction (PCR) Jump Station
information and links on all aspects
of the Polymerase Chain Reaction...
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Merlin purification of genomic DNA
a new simple and inexpensive procedure
in which the pure DNA is extracted from the whole blood
within 15-20 min...
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Anderson's Guides for Biological Reagents...
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Protocols for Biochemistry and Molecular Biology
by John Garland, include agarose gel,
PAGE...
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ScienceXchange Protocols include
molecular Biology, Molecular Immunology, Immunochemistry,
Immunology...
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The Jackson Laboratory IMR Genotyping Protocols...
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Molecular Biology Tech-Online
from Life Technologies, Tech Bulletins,
protocols, FAQs, Troubleshooting Guides...
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Techniques in Molecular Biology
from Boehringer Mannheim Biochemicals...
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Dendrome Molecular Biology Protocols...
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Carthew Lab Manual Drosophila
protocols, including somatic mosaic analysis, mutagenesis
and immunostaining....
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DNA Sequencing Lab Protocols from
Whitehead Institute/MIT Center for Genome Research,
contains protocols for DNA purification and sequencing
methods and M13 library construction from cosmids, BAC's
or P1's.
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Phillip Shelp Customized Biology Laboratory Manuals
includes
PCR Introduction, DNA Work, Plant Work, In Situ Hybridization,
Membrane Transfers
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University of Michigan Transgenic Animal Model Core
- Selected Laboratory Protocols
includes DNA Purification for Microinjection,
Preparation of DNA from Tail Biopsies, PCR Analysis
of Tail DNA, Transgene Copy Standards
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Marcantonio Lab Protocol Manual
contains Alkali Denaturation, Competent
HB-101s, CsCl Gradient, Exonuclease III Deletion, Glassing
Out, Large-scale Plasmid Prep., Minigel
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Applications for PCR, electroporation, microinjection,
nucleic acid purification, from
Brinkmann Instruments.
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, Molecular Biology Protocols from
Kirkegaard, Perry Laboratories, Inc.
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Wilcox Lab
contains protocol on In Situ Hybridization
and course outline on in situ hybridization by Josiah
N. Wilcox.
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DNA Amplification Protocol from
MBI Fermantas includes Guidance in Avoiding Contamination,
Preparation of Reaction Mixture, Reaction Mixture Set
Up
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Goldberg Lab Standard Protocols
includes methods and procedures, media,
buffer and reagent recipies.
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Bart's Cookbook and Lab Protocols
includes Protein Biochemistry, Tissue
Culture
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Laboratory Methods from Dohlman Laboratory
includes Pheromone Halo Assay, GST Fusion
Protein Purification from Yeast, Plasmid Library Amplification,
Liquid b-gal Assays
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Technical Bulletins, Technical Manuals, the Protocols
for Promega Products
listed by application, includes cDNA
synthesis and cloning, cell analysis, enzymes for molecular
biology, eukaryotic transcription regulation, immunological
detection
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The Original CPC: Comprehensive
Protocol Collection on Molecular Biology; Nematode-related,
from Ambros lab, includes molecular biology protocols,
C. elegans protocols, other protocol.
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Tips From the Bench
from Ambion, includes practical tips for handling RNA,
isolation of total RNA from difficult tissues, RNA conversions,
increasing your RNA recovery
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Signal Transduction Laboratory Manual
from the Peter MacCallum Cancer Institute,
includes molecular biology, biochemical techniques,
cell biology.
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Molecular Biology Protocols from Beverly Faulkner-Jones
includes extraction; purification of
RNA, quantitation of nucleic acid concentration, Isolation
of total cellular RNA...
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Protocols on Cell Culture, Molecular Biology, Cell Biology/Immunology,
Plant Research
from Life Technologies.
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Two Hybrid System TRAFO Protocol
from Gietz Lab, the protocol is for
2 Hybrid system screens.
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Protocol for isolation of chromosomal DNA from Mycobacteria
provided by dntf@technologist.com.
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Shortcuts to Molecular Biology Protocols
- Protocol
Online omprehensive
collection of molecular biology protocols, methods and
tips, includes DNA, RNA; protein related protocols,
DNA-protein interaction, plasmid, yeast, basic techniques.
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Immobilon Transfer Membranes: Protein Blotting Applications
Guide
from Millipore - Introduction, Protein
Blotting Applications; Immobilon Membrane Applications
Guide.
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Materials ; Methods: Jim Brown's
Hypercard stack of common molecular biology lab protocols.
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ExPASy Molecular Biology Server at Geneva University
Hospital provides protocolsnd
- Industrial
In Vitro Toxicology Group: In
vitro test Protocols
has a collection of in vitro test protocols,
including liver cell culture and assays.
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Cell and Molecular Biology Online
Educational Resources
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